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Home » News

Researchers at National Eye Institute use 3D bioprinting to create eye tissue

News Press Release By AM Chronicle Editorial TeamDecember 24, 20224 Mins Read
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Scientists used patient stem cells and 3D bioprinting to produce eye tissue that will advance understanding of the mechanisms of blinding diseases. The research team from the National Eye Institute (NEI), part of the National Institutes of Health, printed a combination of cells that form the outer blood-retina barrier—eye tissue that supports the retina’s light-sensing photoreceptors. The technique provides a theoretically unlimited supply of patient-derived tissue to study degenerative retinal diseases such as age-related macular degeneration (AMD).

Scientists used patient stem cells and 3D bioprinting to produce eye tissue that will advance understanding of the mechanisms of blinding diseases.

“We know that AMD starts in the outer blood-retina barrier,” said Kapil Bharti, Ph.D., who heads the NEI Section on Ocular and Stem Cell Translational Research. “However, mechanisms of AMD initiation and progression to advanced dry and wet stages remain poorly understood due to the lack of physiologically relevant human models.”

The outer blood-retina barrier consists of the retinal pigment epithelium (RPE), separated by Bruch’s membrane from the blood-vessel rich choriocapillaris. Bruch’s membrane regulates the exchange of nutrients and waste between the choriocapillaris and the RPE. In AMD, lipoprotein deposits called drusen form outside Bruch’s membrane, impeding its function. Over time, the RPE break down leading to photoreceptor degeneration and vision loss.

 

3d bioprinted eye tissues
The outer blood-retina barrier is the interface of the retina and the choroid, including Bruch’s membrane and the choriocapillaris. (Image: National Eye Institute)

Bharti and colleagues combined three immature choroidal cell types in a hydrogel: pericytes and endothelial cells, which are key components of capillaries; and fibroblasts, which give tissues structure. The scientists then printed the gel on a biodegradable scaffold. Within days, the cells began to mature into a dense capillary network.

On day nine, the scientists seeded retinal pigment epithelial cells on the flip side of the scaffold. The printed tissue reached full maturity on day 42. Tissue analyses and genetic and functional testing showed that the printed tissue looked and behaved similarly to native outer blood-retina barrier. Under induced stress, printed tissue exhibited patterns of early AMD such as drusen deposits underneath the RPE and progression to late dry stage AMD, where tissue degradation was observed. Low oxygen induced wet AMD-like appearance, with hyperproliferation of choroidal vessels that migrated into the sub-RPE zone. Anti-VEGF drugs, used to treat AMD suppressed this vessel overgrowth and migration and restored tissue morphology.

Growth of blood vessels across printed rows of an endothelial-pericyte-fibroblast cell mixture. By day 7, blood vessels fill in the space between the rows, forming a network of capillaries. Image credit: Kapil Bharti.

“By printing cells, we’re facilitating the exchange of cellular cues that are necessary for normal outer blood-retina barrier anatomy,” said Bharti. “For example, presence of RPE cells induces gene expression changes in fibroblasts that contribute to the formation of Bruch’s membrane – something that was suggested many years ago but wasn’t proven until our model.”

eye outer blood retina barrier
The eye’s outer blood-retina barrier comprises retinal pigment epithelium, Bruch’s membrane and the choriocapillaris. (Image: National Eye Institute)
growth of blood vessels
Growth of blood vessels across printed rows of an endothelial-pericyte-fibroblast cell mixture. By day 7, blood vessels fill in the space between the rows, forming a network of capillaries. (Image: Kapil Bharti)

Among the technical challenges that Bharti’s team addressed were generating a suitable biodegradable scaffold and achieving a consistent printing pattern through the development of a temperature-sensitive hydrogel that achieved distinct rows when cold but that dissolved when the gel warmed. Good row consistency enabled a more precise system of quantifying tissue structures. They also optimized the cell mixture ratio of pericytes, endothelial cells, and fibroblasts.

Co-author Marc Ferrer, Ph.D., director of the 3D Tissue Bioprinting Laboratory at NIH’s National Center for Advancing Translational Sciences, and his team provided expertise for the biofabrication of the outer blood-retina barrier tissues “in-a-well,” along with analytical measurements to enable drug screening.

“Our collaborative efforts have resulted in very relevant retina tissue models of degenerative eye diseases,” Ferrer said. “Such tissue models have many potential uses in translational applications, including therapeutics development.”

Bharti and collaborators are using printed blood-retina barrier models to study AMD, and they are experimenting with adding additional cell types to the printing process, such as immune cells, to better recapitulate native tissue.

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Reference: 
3D outer retina barrier uncovers RPE-dependent choroidal phenotype in advanced macular degeneration. Nature methods. https://www.nature.com/articles/s41592-022-01701-1. DOI:10.1038/s41592-022-01701-1.

Original Source

3d printing additive manufacturing biomedical bioprinting eye Healthcare National Eye Institute
AM Chronicle Editorial Team

The AM Chronicle Editorial Team is a collective of passionate individuals committed to delivering insightful, accurate and engaging stories to additive manufacturing audiences worldwide.

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